Coding
FadA

Part:BBa_K861400:Design

Designed by: Kuanwei Sheng   Group: iGEM12_WHU-China   (2012-08-16)


FadA protein coding sequence for fatty acid degradation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
    Illegal BglII site found at 960
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 574
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Before constructing the final system and testing its function, we constructed three biobrick parts with 3-ketoacyl-CoA thiolase activity (FadA, FadI, S-FadA) as candidates.


Source

Escherichia coli K.12 MG1655 genome

References